Here is the link for the video: https://www.youtube.com/watch?v=Xb2eDXJ&pw4 Bovine Scrum Albumin (BSA) Standard solution: 1,0 mg/mL in 33m. I need to determine how much protein I have in my sample;How much sample need to use this method or as both of my sample used in this method?I'm not good in english sorry, but is important for me know that both of my sample can process this method if I have only 1mg can used this method.I explain? Show here how you would prepare the portion of 4 mM working reagent you need to do your assay. I suggest a SDS-PAGE control where you compare a standard protein (BSA?) Assume you will need 50 mL of the 4 mM solution and show in your report how you would prepare that portion of reagent you need to do your experiment. !the method with running a gel with different dilution of the sample is not good for me, since i realy hav a lot of samples for each experimentthanks for help! The universality of the BCA assay is that it is useful for analysis of most any protein because the chemical reaction is independent of the nature of the R-groups. The Standard Protocol permits either a 30-minute incubation at 37°C or a 2 hour-incubation at room temperature. How would results differ if a pure water blank is used instead of a blank prepared with all reagents except for the protein? i use the bca kit from Pierce and it shows that the protein concentration in my sample is very low or none. Prepare BCA working reagent (WR). Module and the BCA Protein Assay is 5 µg/mL (Figure 1) and the maximum is 2 mg/mL (Figure 2). THe sample to working reagent is 1:20 instead of 1:8 in the micro assay. Pipette 25 or 10 μl of each standard or protein sample replicate into a microplate well. Give two or three facts/characteristics of serum albumin. Submit the standard curve showing the best fit straight line for the triplicate data points with error bars based on the data points indicating standard deviations. The Cu 1+ ions then react with bicinchoninic acid (BCA) to form a purple-colored product that absorbs at 562 nm. It makes use of the biuret reaction, in which the protein backbone chelates Cu 2+ ions and reduces them to Cu 1+ ions. An assay" is a test procedure usually based on a reaction that gives a colored product that absorbs light efficiently at discreet wavelengths in the visible region of the optical spectrum. Triplicalc values of Abs at 562 nm are given for the BSA standards. BCA protein assay reagents (Pierce, catalog number: Prepare bovine serum abumin (BSA) standards. 3. What is the purpose of using a "blank" when doing spectrophotometric measurements in an assay?